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| 12th Biology Chapter 23 - Biotechnology Questions Bank |
Biology 2nd Year Chapter 23 Biotechnology Solved MCQs, Important Short Questions and Long Questions Bank
MCQs
Please select the right answer.
1. A full set of genes of an individual is called its:
2. A probe is a single stranded nucleotide sequence that will hybridize in to certain piece of:
3. Adult transgenic tobacco plants glowed when sprayed with the substrate
4. An antibody made by soyabeans can be used as treatment for
5. An enzyme a-galactosidase that can be used to treat a human lysosome storage disease, is harvested from?
6. An undifferentiated group of cells is called
7. Anther is cultured in a medium containing
8. Antibody used for the treatment of genital herpes is obtained from:
9. Antithrombin is produced in
10. Aspartame is a:
11. Biodegradable plastic is obtained from
12. Callous can form
13. Cell suspension cultures of digitalis lanata produce:
14. Children with severe combined immunodeficiency syndrome lack enzyme:
15. Cloning is a form of:
16. Complete carrot plant was developed from tiny part of phloem by
17. Cystic fibrosis patients lack a gene that codes for trans-membrane carrier of the
18. DNA analyzer is used for
19. E.Coli s a commonly used
20. Each piece of DNA should be
21. Enzyme obtained from thermus aquatieus is commonly known as:
22. FC Steward provided the phloem cells
23. Gene pharming is used for obtaining
24. Genes are cut by
25. Genome fragments can be separated according to their lengths during the process:
26. Genomic library is a collection of bacterial or bacteriophage?
27. Green revolution was launched in
28. Growth of a tissue in an artificial liquid culture medium is termed as:
29. In 1958, F.C.Steward grew a complete carrot plant from tiny piece of:
30. In cystic fibrosis, the gene codes for trans membrane carrier of which ion is missing
31. In gene pharming egg is fertilized
32. In gene sequencing the nucleotide strained with fluorescent dye are
33. In gene sequencing, the colour of the fluorescent bands are read by
34. In micropropagation of meristem, the hormone used is
35. In micropropagation, the structure used is
36. In the cloning of mice the second nucleus is obtained from
37. In which of the followings plant pest and herb resistant genes are not used?
38. In which of these in vivo gene therapy is used?
39. Kary B.Mullie developed the polymorase chain reaction in
40. Match MaxamGilbert method for generation of pieces of DNA with one of the followings
41. Match recombinant DNA with one of the followings
42. Match Sanger’s method for generation of pieces of DNA with one of the followings
43. Match target DNA with one of the followings
44. Mendel’s work was rediscovered in
45. Meristem is
46. Millions of artificial seed are produced in large tanks called
47. Natural extra chromosomal circular DNA are
48. Naturasweet is
49. Number of restriction enzymes discovered so far are
50. Organisms that have had a foreign gene inserted into them are called
51. Organisms with foreign genes are called
52. Palindromic sequences are present in
53. Patients of cystic fibrosis often die due to numerous infections of the:
54. pBR 322 has antibiotic resistance genes for:
55. PCR technique was developed by
56. Persons with Huntington's disease have a unique site where a restriction enzyme cuts:
57. Pieces of DNA are jointed by
58. Pieces of DNA are separated on
59. Production of identical copying of gene is
60. PSc 101 has antibiotics resistant gene for
61. Recombinant DNA are expressed in
62. Restriction enzymes are present in
63. Scientists are working on which of followings for increasing yield
64. Somatic embryo are encapsulated in gel and are called
65. Taq polymarase is an enzyme present in
66. The acreage of transgenic plants in 1999 was
67. The antibody which can deliver radio isotopes to tumor cells is obtained from
68. The antibody which is used for the treatment of genital herps is obtained from
69. The artificial seeds are produced of which of the following plants?
70. The bacteria are used in mining industry for
71. The bacteria used in industries for control of pollution are
72. The cell suspension cultures of Cnchona ledgeriana produces:
73. The chemical that makes the bacterial membrane permeable is
74. The clumps produce
75. The coconut milk contains the plant hormone called:
76. The collection of different sized fragments is
77. The commonly used restriction enzyme is
78. The copy of DNA pieces in human genomic project is made by
79. The degradation of pollutants with the help of recombinant bacteria is called
80. The disease not treated by Ex vivo gene therapy is
81. The DNA sequence of which of the followings have been done?
82. The entire genome has been sequence by the company of
83. The enzyme ADA is involved in maturation of
84. The enzyme extracted from Thermus aquaticus is
85. The enzyme luciferase is produced by an insect commonly known as the
86. The enzymes which are used to cut out the gene of interest are known as:
87. The first animal cloned was
88. The first restriction enzyme was isolated by
89. The first restriction enzyme was isolated by
90. The gene sequence of which of the following chromosomes was completed in 1999
91. The gene therapy in cancer makes the patient tolerant for
92. The genes for the treatment of hypercholesterolemia are introduced in
93. The genes for the treatment of SCID are introduced in
94. The genes which are synthesized from mRNA by reverse transcriptase are called
95. The genes which cause self destruction of bacteria are
96. The human genomic project will take how many volume of encyclopedia
97. The hypercholesterolemia causes at young age
98. The insertion of genetic material into human cells for the treatment of a disorder is called
99. The microscopic vesicles formed during gene therapy of cystic fibrosis are called
100. The naked cells of plant are called
101. The nucleotide sequence is stored by
102. The number of base sequences in man is
103. The number of frequently used restriction enzymes are
104. The organism used as vector during treatment of SCID by gene therapy is
105. The organisms which are used to produce human growth hormone in their urine are
106. The plant cells whose cell wall is removed are
107. The plants cells can develop complete plants.So they are called
108. The plants produced by micropropagation from single cell are called
109. The plants produced by micropropagation of meristem are called
110. The plasmids having resistant gene against tetracycline and ampicillin are
111. The production of genetically identical copies of the organisms by asexual reproduction is called
112. The protoplast regenerates and produces
113. The single standard nucleotide sequence that hybridize into certain piece is
114. The smallest human chromosomes is
115. The solution of Liposomes is sprayed on
116. The steps involved in DNA finger printing
117. The system used for the formation of specific product is
118. The technique used to produce copies of genes in laboratory is
119. The two different piece of DNA joined together, is called as
120. The use of DNA analysis for convicting the criminal of rape is used in
121. The use of transgenic farm animals to produce pharmaceuticals is termed as:
122. The work of biotechnology is
123. Thermus aquateua is a/ an
124. These are used in angiosplasty
125. Tissue culture and cloning seek help through:
126. Transgenic bacteria are produced in large vats called
127. Urine is a prefereable vehicle for bilotechnology product than:
128. Which of the enzymas sot as molecular scissors
129. Which of the following diseases is not treated by in vivo gene therapy?
130. Which of the following is virus free?
131. Which of the following phages is use as vector?
132. Which of the following products is prepared by recombinant DNA technology?
133. Which of the following techniques produces large number of copies of genes?
134. Which of the followings form genomic library?
135. Which of the followings helped scientist to pinpoint disease causing genes?
136. Which of the followings is irrelevant for PCR?
137. Which of the followings is model plant whose gene sequencing has been completed?
138. Which of the followings is not a vector?
139. Which of the followings is produced by transgenic plants as well as transgenic bacteria?
140. Which of the followings is used to transfer genes?
141. Which one is not biotechnology product?
Short Questions
1. Define angioplasty.
2. Define biotechnology.Give its application.
3. Define gene therapy?
4. Define Molecular scissors. What are restriction enzymes? Give example. Differentiate between molecular scissors and molecular vectors?
5. Define plasmids?
6. Define tissue culture?
7. Describe genomic library.
8. Differentiate between Lucifer in and Luciferase?
9. Explain cell suspension culture?
10. Explain familial hypercholesterolemia?
11. Explain restriction enzyme (restriction endonuclease)?
12. Explain the importance of gene sequencing.
13. From which amimal antithrombin III is produced?
14. Give definition of hybridization?
15. Give examples of ex-vivo and in-vivo gene therapies in man?
16. Give two advantages of transgenic animals?
17. Give two advantages of transgenic plants?
18. How can hypercholesterolemia be controlled by gene therapy?
19. How can Parkinson’s disease be cured by gene therapy?
20. How can the useful (desirable) genes be obtained for producing recombinant DNA?
21. How gene therapy has been used for the treatment of cancer?
22. How gene therapy has been used for the treatment of coronary artery angioplasty?
23. How genes can be isolated from chromosomes?
24. How is the enzyme, DNA ligase used in genetic engineering?
25. How many methods are used for gene therapy?Name them.
26. How transgenic animals are developed?
27. How transgenic animals that secrete a product are often cloned?
28. Name different organisms and organelles whose genomes have been sequenced?
29. Name some biotechnology products produced by bacteria?
30. Name the enzyme which helps synthesize desirable gene in the laboratory, form mRNA.
31. plant cells are said to be totipotent.What do you mean by this?
32. What are Biofilters?
33. What are bioreactors?
34. What are biotechnology products?
35. What are clonal plants?
36. What are Palindromic Sequences?
37. What are plasmids?
38. What are protoplasts?
39. What are sticky ends?
40. What are teh restriction enzymes?
41. What are the various methods of gene or DNA sequencing?
42. What are transgenic organisms(animals or plants)?
43. What are transgenic plants. Give two advantages of transgenic plants.
44. What are various methods of gene or DNA sequencing?
45. What are vectors?
46. What can be used as vector, other than the plasmid?
47. What do you mean by palindromic (repeated) sequences?
48. What does genome mean?
49. What is a genome and genomic library?
50. What is a genome and genomic library? Differentiate between genome and genomic library. Define genomic library.
51. What is a probe?
52. What is a protoplast?
53. What is another name for recombinant DNA?
54. What is aspartame?
55. What is clone?
56. What is complementary DNA?
57. What is cystic fibrosis?
58. What is DNA Finger printing?
59. What is Dolly?
60. What is Ex-vivo gene therapy?
61. What is familial hypercholesterolemia?
62. What is gel electrophoresis?
63. What is gene pharming?
64. What is gene sequencing?
65. What is gene therapy?
66. What is in-vivo gene therapy?
67. What is Maxam-Gilbert method?
68. What is meant by cloning?
69. What is PCR and write applications of PCR amplification. What are the uses of PCR amplification and analysis?
70. What is recombinant DNA and its technology in genetic engineering?
71. What is recombinant DNA or chimaeric DNA?
72. What is Sanger’s method?
73. What is the advantage of genetic engineering of C4 plants?
74. What is the biodegradable plastic and its origin?
75. What is the method of ex-vivo gene therapy?
76. What is the polymerase chain reaction of PCR?
77. What is the purpose of primary goal of human genome project?
78. What is the role of molecular carrier-the vector? Differentiate between plasmids pSC 101 and pBR 322? Elaborate the use of plasmids. Mention the role of lambda phage during recombinant DNA technology.
79. What is the role of restriction enzyme in the preparation of recombinant DNA during genetic engineering?
80. What is the role of suicide gene in transgenic bacteria?
81. What is the use of dideoxy method?
82. What is the use of DNA or chimaeric DNA?
83. What is the use of genetically engineered bacteria?
84. What is tissue culture?
85. What is totipotency?
86. What is totipotency? What is totipotent cell? Define the term totipotent. Why plant cells are said to be totipotent?
87. What is vector?
88. When recombinant DNA technology is used and when PCR?
89. Where from PCR took its name?Why is callled chain reaction?
90. Which chemicals are obtained from cell suspension cultures of Cinchona ledger Lana and Digitalis lanata?
91. Which enzyme is Taq polymerase?
92. Which plant hormone is contained in the coconut milk?
93. Which resistance genes are carried by the plasmids pSC 101 pBR 322?
94. Who and when grew a whole carrot plat from a tiny piece of phloem?
95. Who and when said that the plant cells are totipotent?
96. Why transgenic animals are cloned?
Long Questions
1. Write a note on tissue culture and cloning.
2. Write a note on tissue culture technique?